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Action of Antimicrobial Copper on Bacteria and Fungi Isolated from Commercial Poultry Hatcheries Rev. Bras. Ciênc. Avic.
Depner,RFR; Pontin,KP; Depner,RA; Flores Neto,A; Lucca,V; Lovato,M.
ABSTRACT Since 2008, when the US Environmental Protection Agency (EPA) registered copper and its alloys as an antimicrobial agent for contact surfaces, research has demonstrated their antimicrobial activity. The aim of this study was to evaluate the efficacy of antimicrobial copper against bacteria and fungi isolated from commercial poultry hatcheries in order to develop a microbiological control alternative in these environments. Samples were collected from the surfaces of hatcher baskets from two hatcheries. Mesophilic microorganisms and fungi/yeasts were isolated and standardized in concentration of 105 cells/mL. Four copper plates and four stainless steel plates were completely immersed for one minute in bacteria and fungi/yeasts solutions and left to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial copper; Hatchery; Stainless steel; Copper surfaces.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000600095
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Adhesion and biocides inactivation of Salmonella on stainless steel and polyethylene BJM
Tondo,Eduardo Cesar; Machado,Taís Raquel Marcon; Malheiros,Patrícia da Silva; Padrão,Débora Kruger; Carvalho,Ana Lyl de; Brandelli,Adriano.
The adhesion of Salmonella (S.) strains to stainless steel and polyethylene and their inactivation by biocides used in food industry was investigated. Coupons of stainless steel and polyethylene were immersed in bacterial suspensions of S. Enteritidis, S. Typhimurium, and S. Bredeney during 15, 30, and 60 minutes, and submitted to different concentrations of peracetic acid (PAA), sodium hypochlorite (NaOCl), and quaternary ammonium (Quat) sanitizers. Hydrophobicity of the surfaces was evaluated by contact angle measurements using the sessile drop method and bacterial adhesion was accompanied through bacterial counts and scanning electron microscopy (SEM). Results indicated that the three serovars of Salmonella presented similar adhesion to both materials...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Adhesion; Biocides; Stainless steel; Polyethylene.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400022
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Bacterial adherence to different inert surfaces evaluated by epifluorescence microscopy and plate count method BABT
Parizzi,Silvânia Quintão Fontes; Andrade,Nélio José de; Silva,Cleuber Antonio de Sá; Soares,Nilda de Fátima Ferreira; Silva,Eldo Antônio Monteiro da.
The adherence of Listeria inoccua L6a and Staphylococcus aureus ATCC6538 was evaluated on stainless steel (SS), polycarbonate (PC) and polypropylene (PP) chips. The EP results showed a significant difference (p<0.05) among the microorganism species interactions, the surfaces and the contact time. The number of adhered cells on the chip surfaces increased as contact time increased and the number of L. innocua or S. aureus on the surface of SS, PC and PP chips reached 1.0x10(5) CFU/cm² after 12 h of contact, in both methods. The CP method showed a better sensitivity to detect low number of adhered cells. The EP was better when the average number of adhered cells was between 10 and 100 per microscopy field.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial adherence; Listeria innocua and Staphylococcus aureus; Stainless steel; Polycarbonate and polypropylene.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100011
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Contribution of environmental factors in the formation of biofilms by Alicyclobacillus acidoterrestris on surfaces of the orange juice industry Ciência Rural
Dutra,Tatiane Viana; Prado,Daniela Biral do; Anjos,Márcia Maria dos; Machinski Junior,Miguel; Mikcha,Jane Martha Graton; Abreu Filho,Benício Alves de.
ABSTRACT: The objective of this study was to evaluate the effect of the initial microbial load, temperature and contact time on the biofilm formation of Alicyclobacillus acidoterrestris on stainless steel and natural food-grade rubber using orange juice as culture medium. The low initial load of A. acidoterrestris (2 log CFU/mL) led to biofilm formation on the stainless steel surface after 48 h of contact at 28 ºC and after 24 h at 45 ºC, and on natural food-grade rubber surface after 48 h of contact at both temperatures. The high initial microbial load (5 log CFU/mL) led to biofilm formation on stainless steel after 4 h of contact at 28 °C and 45 °C, while biofilm was formed on natural food-grade rubber after 8 h of contact at 28 °C and 4 h at 45 °C. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Concentrated orange juice; Stainless steel; Natural food-grade rubber; Spores; Biofilm.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000300753
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Electroactive Bacteria Associated With Stainless Steel Ennoblement in Seawater ArchiMer
Trigodet, Florian; Larche, Nicolas; Morrison, Hilary G.; Jebbar, Mohamed; Thierry, Dominique; Maignien, Lois.
Microorganisms can increase the open-circuit potential of stainless steel immersed in seawater of several hundred millivolts in a phenomenon called ennoblement. It raises the chance of corrosion as the open-circuit potential may go over the pitting corrosion potential. Despite the large impact of the ennoblement, no unifying mechanisms have been described as responsible for the phenomenon. Here we show that the strict electrotroph bacterium "Candidatus Tenderia electrophaga" is detected as an ennoblement biomarker and is only present at temperatures at which we observe ennoblement. This bacterium was previously enriched in biocathode systems. Our results suggest that "Candidatus Tenderia electrophaga," and its previously described extracellular electron...
Tipo: Text Palavras-chave: Ennoblement; Electroactive bacteria; Stainless steel; Microbial ecology; 16S rRNA gene.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00482/59371/62144.pdf
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Fonctionnalisation de surfaces d'acier inoxydable par des enzymes en vue d'inhiber l'adhésion de bactéries et la formation de biofilms en eau de mer ArchiMer
Caro, A.; Humblot, V.; Minier, M.; Salmain, M.; Compère, Chantal; Pradier, C.-m..
All surfaces that are immersed in seawater rapidly become covered by nitrogenous compounds, proteins, carbohydrates, salts, silica and bacteria in a few hours, and by a grown biofilm in a few days. These biodeterioration, physical and chemical damages involve important cleaning costs. Organostanic paintings used up to now to protect the surfaces against this spoiling will be prohibited in 2008 because of their toxicity. That's why the stake today is to find alternatives to these paintings. The solution which is here suggested consists in acting on the first stages of the formation of the biofilm i.e. the proteins and bacterial adsorption on the surface. For this, the surface is chemically modified then functionalized by enzymes. Enzymes will act locally on...
Tipo: Text Palavras-chave: Acier inoxydable; Fonctionnalisation; Enzymes; Adsorption bactérienne; PM-IRRAS; XPS; Stainless steel; Functionalization; Enzymes; Bacterial adsorption; IR; XPS.
Ano: 2006 URL: https://archimer.ifremer.fr/doc/00479/59095/61733.pdf
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Formation of biofilms by Staphylococcus aureus on stainless steel and glass surfaces and its resistance to some selected chemical sanitizers BJM
Marques,Simone Cristina; Rezende,Jaíne das Graças Oliveira Silva; Alves,Lizandra Aparecida de Freitas; Silva,Belami Cássia; Alves,Eduardo; Abreu,Luiz Ronaldo de; Piccoli,Roberta Hilsdorf.
The objectives of this work were to verify the capability of Staphylococcus aureus of forming bio-film on stainless steel and glass surfaces; to evaluate the efficiency of sodium dichloroisocyanurate, hydrogen peroxide and peracetic acid in inactivating Staphylococcus aureus cells adhered onto these surfaces; and to visualize biofilm development by scanning electron microscopy before and after sanitizer treatment. The surfaces studied consisted of 10x20mm chips immersed in Petri dishes containing BHI broth inoculated with S. aureus ATCC 25923. Biofilm formation was observed after 15-day incubation, when the cells were removed using the swab technique, followed by Baird Parker agar plating. Also, the efficiency of the chemical sanitizers on the chip...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilm; Stainless steel; Glass; Staphylococcus aureus; Sanitizers; Scanning electron microscopy.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300029
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Influence of dissolved oxygen content on the bacteria‐induced ennoblement of stainless steels in seawater and its consequence on the localized corrosion risk ArchiMer
Trigodet, Florian; Larché, Nicolas; Morrison, Hilary G.; Maignien, Lois; Thierry, Dominique.
The ennoblement of stainless steel (e.g., the increase of open circuit potential [OCP]) is associated with bacterial colonization. This increases the risk of localized corrosion as the critical pitting/crevice potential can be overcome, especially for lower grade stainless steel. In this study, we assessed the influence of dissolved oxygen content (DOC) on the crevice corrosion of duplex and super duplex stainless steels. In addition, we used DNA amplicon sequencing to identify the bacteria most likely associated with the ennoblement. Above approximately 100 parts per billion (ppb) of dissolved oxygen, the ennoblement of OCP was observed leading to an increased risk of localized corrosion. Below approximately 100 ppb of dissolved oxygen, no ennoblement...
Tipo: Text Palavras-chave: 16S rRNA gene; Electroactive bacteria; Ennoblement; Microbial ecology; Natural seawater; Stainless steel.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00588/70026/67934.pdf
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Sanitizer resistance of biofilm-forming Salmonellaisolated from meat products Arq. Bras. Med. Vet. Zootec.
Milan,C.; Agostinetto,A.; Conceição,R.C.S.; Gonzalez,H.L.; Timm,C.D..
Este estudo avaliou a capacidade de Salmonella enterica subsp. enterica isolada de produtos cárneos formar biofilme e testou sua resistência a diferentes sanitizantes. Vinte cepas foram avaliadas quanto à capacidade de formar biofilme em placas de microtitulação. As cepas formadoras de biofilme foram testadas em superfícies de polietileno de alta densidade, aço inoxidável e vidro e tiveram a sensibilidade ao hipoclorito de sódio e ao iodo avaliada. Duas cepas de Salmonella Enteritidis isoladas de produtos de frango apresentaram capacidade de formar biofilme nas superfícies testadas. Essas cepas alcançaram maiores populações nas superfícies do que aquelas não formadoras de biofilme, e foram mais difíceis de remover ou reduzir. Devido à ação sanitizante ser...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Biofilm; Salmonella; Glass; Stainless steel; High-density.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000200642
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Single- and Multispecies Biofilms by Escherichia coli, Staphylococcus aureus, and Salmonella spp. Isolated from Raw Fish and a Fish Processing Unit Ciência Rural
Frozi,Jesieli Braz; Esper,Luciana Maria Ramires; Franco,Robson Maia.
ABSTRACT: The consumption of fish by the Brazilian population is increasing. However, fish and seafood are highly perishable and can be contaminated with several microorganisms. In addition, the possibility of biofilm formation is a greater cause for concern. In this study, biofilm formation was evaluated in single- and multispecies cultures at 25°C for incubation periods of 0, 4, 8, 24, and 48h in stainless steel coupons (size, 1.0×1.0cm) immersed in tryptic soy broth. The characteristics of the formed biofilms after sanitizing by immersing the coupons in 200ppm sodium hypochlorite solution for 10min were also evaluated under the same experimental conditions but with some modifications. Biofilm structure was evaluated using scanning electron microscopy....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella spp.; Escherichia coli; Staphylococcus aureus; Biofilm; Stainless steel; Fish industry..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001000451
Registros recuperados: 10
Primeira ... 1 ... Última
 

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